2009.
Sato Y, Nabeta Y, Tsukahara T, Hirohashi Y, Syunsui R, Maeda A, Sahara H, Ikeda H, Torigoe T, Ichimiya S, Wada T, Yamashita T, Hiraga H, Kawai A, Ishii T, Araki N, Myoui A, Matsumoto S, Umeda T, Ishii S, Kawaguchi S, Sato N (2002) Detection and induction of CTLs specific for SYT-SSX-derived peptides in HLA-A24(+) patients with synovial sarcoma. Thank you for visiting nature.com. Wan G, Zhaorigetu S, Liu Z, Kaini R, Jiang Z, Hu CA (2008) Apolipoprotein L1, a novel Bcl-2 homology domain 3-only lipid-binding protein, induces autophagic cell death. Genito Urinary Cancer Research Group, School of Cancer and Imaging Sciences, Paterson Institute for Cancer Research, University of Manchester, Manchester, M20 4BX, UK. Warner JK, Wang JC, Hope KJ, Jin L, Dick JE (2004) Concepts of human leukemic development. First, TGF‐β and TGF‐β receptor are overexpressed in pancreatic cancer tissue.Finally, the present study demonstrates that SP cells of pancreatic cancer cell lines exert increased The authors acknowledge Dr. Gregory J. Gores, Mayo Clinic, Rochester, Minnesota, for his stimulating and suggestive discussion. Stem Cells. Side population (SP) cells are a rare subset of cells, which can be isolated by dual-wavelength flow cytometry because of their capacity to efflux Hoechst 33342, found in various tissues and cell lines that are highly enriched for stem cell activity. Detection of side population cells in bone sarcoma cell lines. On day 14, SP cells showed spherical colony formation (To address the issue of whether tumourigenic activity differed between SP and MP cells, 1 × 10Finally, we performed gene expression profiling of SP cells using cDNA microarrays. We specifically focused on their malignant characteristics regarding the process of metastasis, including micro‐invasion and EMT. Analyzed SP fraction in pancreatic cancer cell lines.
You can also search for this author in These results suggest that SP cells have superior potential to up‐regulate E‐cadherin expression.
Singh SK, Hawkins C, Clarke ID, Squire JA, Bayani J, Hide T, Henkelman RM, Cusimano MD, Dirks PB (2004) Identification of human brain tumour initiating cells. A total of 2000 SP and MP cells were sorted and cultured immediately under conditions of serum starvation, providing an anchorage-independent environment.
You can also search for this author in Tsukahara T, Kawaguchi S, Torigoe T, Kimura S, Murase M, Ichimiya S, Wada T, Kaya M, Nagoya S, Ishii T, Tatezaki S, Yamashita T, Sato N (2008a) Prognostic impact and immunogenicity of a novel osteosarcoma antigen, papillomavirus binding factor, in patients with osteosarcoma. The dye-labelled aRNA samples were hybridised to a 29 138-spot Human Panorama Micro Array (Sigma-Aldrich) for 16 h at 45°C.
Zhou S, Morris JJ, Barnes Y, Lan L, Schuetz JD, Sorrentino BP (2002) Bcrp1 gene expression is required for normal numbers of side population stem cells in mice, and confers relative protection to mitoxantrone in hematopoietic cells We thank Ms Keiko Miwa (Team for Cell Lineage Modulation, RIKEN Center for Developmental Biology, Kobe, Japan) for her technical support. Cancer Res … Primers were designed to generate a PCR product of <200 bp.
Szotek PP, Pieretti-Vanmarcke R, Masiakos PT, Dinulescu DM, Connolly D, Foster R, et al. Perez-Pinera P, Berenson JR, Deuel TF (2008) Pleiotrophin, a multifunctional angiogenic factor: mechanisms and pathways in normal and pathological angiogenesis. genes by mitogen-activated protein kinase/extracellular signal-regulated kinase pathway and its role in generation of side population cells in canine lymphoma cell lines As shown in The re-population of SP cells into both SP and MP cells. 6-9 SP cells are detected by their own ability to efflux Hoechst 33342 dye through an adenosine triphosphate (ATP)‐binding cassette (ABC) membrane transporter. To further evaluate EMT‐associated gene alterations, we next observed the mRNA levels of E‐cadherin, snail, MMP‐2 by real‐time PCR. (To examine whether SP cells could generate both SP and MP cells, sorted SP and MP cells were further cultured We next evaluated the ability of SP and MP cells to generate spherical colonies. View Article: Google Scholar: PubMed/NCBI. Ovarian cancer side population defines cells with stem cell-like characteristics and Mullerian Inhibiting Substance responsiveness. All media were supplemented with 10% fetal bovine serum and 100,000 units per liter penicillin, 100 mg/l streptomycin (GIBCO).To identify and isolate the SP cell fraction and main population (MP) cell fraction, cells were removed from the culture dish with trypsin and EDTA, washed, suspended at 1 × 10Invasion assay was performed using Biocoat Cellwere (Becton Dickinson). As depicted in Detection of side population cells in bone sarcoma cell lines. Hirschmann-Jax C, Foster AE, Wulf GG, Nuchtern JG, Jax TW, Gobel U, Goodell MA, Brenner MK (2004) A distinct ‘side population’ of cells with high drug efflux capacity in human tumor cells. (A) The populations of SP cells of seven osteosarcoma cell lines (NY, OS2000, KIKU, … However, E‐cadherin expression was dramatically up‐regulated in SP cells within a few days of culturing.
Ho MM, Ng AV, Lam S, Hung JY (2007) Side population in human lung cancer cell lines and tumors is enriched with stem-like cancer cells.
Cells were pre‐treated with TGF‐β for 3 days.
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